Electrophoresis


CLEAVER SCIENTIFIC LTD is an innovative leader in the design and manufacturing of electrophoresis equipment and related products 

A long-established technique, horizontal gel electrophoresis offers many advantages for nucleic acid separation, and remains today one of the mainstays of molecular biology. The Cleaver Scientific MultiSUB ™ range offers the most comprehensive and versatile range of horizontal gel electrophoresis units currently available for low- and high-throughput DNA and RNA applications and have been designed by scientists with the laboratory scientist in mind.

  • Durable and leak-proof tank design for complete safety and longevity
  • Cassette-style electrodes are difficult to break, but inexpensive and easy to change
  • The widest range of combs available of any gel tank manufacturer
  • Multiple gel tray options eliminate the need for additional gel tanks and allow gels to be cast externally, keeping the tank permanently in use for electrophoresis
  • ‘Plug-and-go’ casting – moulded casting dams clip easily onto the ends of the gel tray for rapid external casting
  • Red loading guides aid well and sample visualisation during loading


Vertical electrophoresis utilises potent protein and nucleic acid analytical tools for applications within all aspects of life science research, ranging from purity determination to analysis of complex protein lysates. Accordingly, Cleaver Scientific remit is simple: to provide a comprehensive range of vertical electrophoresis systems – complete with tanks, inserts and reagents – to fulfill a variety of applications and techniques in different gel sizes and sample capacities

The omniPAGE Mini, Mini Wide and new VS20WAVE Systems each comprise of a modular tank design with optional dedicated inserts available for PAGE, blotting and capillary gel isoelectric focusing (IEF), potentially allowing the user to perform up to three techniques in a day.

  • Corrosion-resistant, 99.99% pure platinum electrodes
  • Elongated thumb locators – colour-coded to prevent polarity reversal and facilitate ‘easy-off’ lid removal
  • Reversible combs also serve as loading indicators to aid pipette-well alignment – preventing loading errors
  • Dual-purpose PAGE inserts eliminate time-consuming transfer of glass plates between separate casting and running modules
  • Cam-Pin caster locks PAGE insert onto the ultra-soft silicone mat within the casting base to provide a leak-free seal
  • 2D & blotting options available


Clinical Electrophoresis Cellulose acetate electrophoresis is an important technique in clinical diagnostics. Cleaver Scientific CellasGEL range is a complete system solution for research and clinical cellulose acetate electrophoresis applications. The CellasGEL range includes both equipment and consumables to assist in the research and diagnosis of specific disease states. This compact high resolution system for clinical electrophoresis can accommodate strips & gels up to 24x20cm and has a complete range of cellulose and acetate gels and kits available as well as Densitometer software and scanner.

 

Blotting is a technique that entails immobilisation of proteins or nucleic acids on a solid membrane support, and then detection using a specific antibody or probe of complementary nucleic acid sequence, blotting significantly increases the potential for identification and characterisation of proteins and nucleic acids. Upon transfer to a membrane, support proteins and nucleic acids become far more accessible to detection by antibodies and probes than they would otherwise be within a gel. Size-fractionation by gel electrophoresis followed by blotting is an excellent way to identify specific molecules within a mixed population of nucleic or protein molecules, and these techniques are often used in tandem. Also available from Cleaver Scientific.

 

What is electrophoresis?

Electrophoresis is an electrokinetic process which separates charged particles in a fluid using a field of electrical charge. It is most often used in life sciences to separate protein molecules or DNA and can be achieved through several different procedures depending on the type and size of the molecules. The procedures differ in some ways but all need a source for the electrical charge, a support medium and a buffer solution. Electrophoresis is used in laboratories for the separation of molecules based on size, density and purity.

An electric field is applied to molecules and as they are electrically charged themselves it results in a force acting upon them. The greater the charge of the molecule the greater the force applied by the electrical field and therefore the further through the support medium the molecule will move relative to its mass.

Some example applications of electrophoresis include DNA and RNA analysis as well as protein electrophoresis which is a medical procedure used to analyse and separate the molecules found in a fluid sample (most commonly blood and urine samples).

Types of electrophoresis

Different types of gels are usually used as the support medium for electrophoresis and this may be in slab or tube form depending on which is more beneficial. Gel slabs enable many samples to be run simultaneously and so are frequently used in laboratories. However, tube gels give a better resolution of the results so are often chosen for protein electrophoresis.

Agarose gel is commonly used for electrophoresis of DNA. It has a large pore structure allowing larger molecules to move easily but it is not suitable for sequencing smaller molecules.
Polyacrylamide gel electrophoresis (PAGE) has a clearer resolution than agarose gel making it more suitable for quantitative analysis. This makes it possible to identify how proteins bind to DNA. It can also be used to develop the understanding of how bacteria is becoming resistant to antibiotics through plasmid analysis.

2D Electrophoresis separates molecules along an x-axis and a y-axis – one separating them by charge and the other by size.