Real Time PCR

Techne Prime Pro 48 Real-time qPCR machine

Made in the UK, the Techne Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.

High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimised 40 cycle reaction down to only 15 minutes. qPCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.

The capacity of Prime Pro 48 accommodates a unique and economical 48-well qPCR plate. The well format mirrors a 384-well plate therefore allowing the use of 16-channel pipettes. The small qPCR plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity and greatly reduces run times.

PCR Max Thermal Cycler ALPHA

With the option for either single, dual or four block instruments throughput and scale are never a concern. The Alpha Cycler 1, 2 and 4 run an HD Quad Core Android tablet interface with features aimed to enhance the user experience and build quality to become the workhorse of any lab.
The PCRmax Alpha cyclers are developed to deliver not only the best quality data you can expect from PCRmax but also to innovate and exceed the high standards expected by the community.

Alpha Cycler 1 PCRmax The single block Alpha Cycler 1 (AC-1) is a compact and feature driven entry level PCR machine with ease of use and performance at its core.

Alpha Cycler 2 PCRmax The Alpha Cycler 2 (AC-2) dual block system allows multiuser labs without the throughput or demand for a four block system access to multi-block flexibility or labs outgrowing their single block system to ramp up their PCRs

Alpha Cycler 4 PCRmax The Alpha Cycler 4 (AC-4) is the only true fully independently controllable multi-block thermal cycler available today. PCRmax developed this system not only with the high throughput market in mind but also for standard throughput and multiple user environments who are space conscious and have requirements for multiple users and block formats.
 
PCR Max Thermal Cycler DELTA kits
 
The Delta Seek detection kits utilise the sensitivity and speed of qPCR to get the most accurate data as quickly as possible. Each kit is specifically designed by our bioinformatics team to ensure the broadest possible detection profile and detection of all clinically relevant strains and subtypes. All test kits are validated in house on multiple qPCR platforms to ensure cross platform functionality.

Kits come with sufficient controls to reduce the chances of making the wrong call and positive copy number standard to allow for easy quantification.

Delta Seek kits can also by generated specifically for your test of interest. If the kit is not in our database currently we can easily make the kit you wish if sequence data is available.

PCR Max Thermal Cycler ECO  
 
The PCRmax Eco 48 utilises patented block and optical technologies to deliver extreme speed, sensitivity and data quality, with optimised protocol as fast as 15 minutes, all using standard plasticware and consumables, sensitivity down to 1 copy detection and the world most accurate block the Eco 48 will deliver Speed. Confidence. Performance. Value and Sensitivity.

The PCRmax Eco 48 real time PCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5µl is validated, cutting reagent costs in half and making more efficient use of expensive and hard to acquire template DNA samples.

Technical Specification


 

High Resolution Melt Yes
Volume per well Validated for 5 to 20 ul
Detection sensitivity 1 copy
Temp uniformity +- 0.1°C
Temp accuracy +- 0.15°C @ 60°C and 95°C
Temperature range 30 to 100 degC
Average ramp rate 5.5 degC sec
Thermal system Hollow silver block peltier-based with conductive fluid
Block format 48-well block
Consumables 48-well custom plates and optical adhesive seals
Optical system Dual LED excitation (452 to 486 nm and 542 to 582 nm)
Camera CCD camera
Filters 4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)
Calibrated dyes SYBR FAM HEX ROX Cy5
Additional dyes All dyes within range compatible with no additional calibration
Passive reference dyes Use of ROX is supported but optional
Data collection Data always collected in all four filters for all wells
Plate setup Plate setup for data analysis can be altered after run completes
Melt curve Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
PCR cycle time (unoptimised) 40 cycles in less than 40 minutes
PCR cycle time (FAST optimised) 40 cycles in less than 20 minutes
Dynamic range Greater than 9 logs
Calibration Not required
Installation Plug and play design
Precision Discriminates 5000 and 10000 template copies with 99 percent confidence
Warranty 1 year (parts and labour included)
Voltage 100 to 240 V.AC
Frequency 50 and 60 Hz
Nominal current draw 5A
Peak power 500W (typical power is 180W)
Software Multiple license software included at no additional cost
Chemistry All chemistries supported
Applications Absolute Quantification and Relative Quantification
Applications Allelic Discrimination and High Resolution Melt
Dimensions closed (WxDxH) 34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)
Dimensions open (WxDxH) 34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)
Weight 13.6 Kg (30 lb)