Real Time PCR qPCR

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Real time PCR

Eco 48 Real time PCR machine PCRmax

The PCRmax Eco 48 utilises patented block and optical technologies to deliver extreme speed, sensitivity and data quality, with optimised protocol as fast as 15 minutes, all using standard plasticware and consumables. With sensitivity of detection down to 1 copy and the worlds most accurate block the Eco 48 will deliver Speed. Confidence. Performance. Value and Sensitivity.

The PCRmax Eco 48 real time PCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5μl is validated, cutting reagent costs in half and making more efficient use of expensive and hard to acquire template DNA samples.

PCRmax qPCR Brochure HERE

 

 Techne Prime Pro 48 Real-time qPCR machine

The Prime Pro 48 real time qPCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.

  • 40 cycles in 40 minutes before optimisation.
  • 40 cycles in 15 minutes when optimised.
  • Genotype in 4 minutes with over 99% accuracy.
  • Set up a 4plex reaction in only 4 mouse clicks.

Techne Prime Pro qPCR Brochure

 

SKU# ECO

The PCRmax Eco 48 utilises patented block and optical technologies to deliver extreme speed, sensitivity and data quality, with optimised protocol as fast as 15 minutes, all using standard plasticware and consumables, sensitivity down to 1 copy detection and the world most accurate block the Eco 48 will deliver Speed. Confidence. Performance. Value and Sensitivity.

The PCRmax Eco 48 real time PCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5µl is validated, cutting reagent costs in half and making more efficient use of expensive and hard to acquire template DNA samples.

The Eco 48 workflow is based on three simple steps:

  • Load samples into the 48-well plate using the backlit Eco Dock and place the plate into the Eco 48.
  • Run experiment.
  • The Eco 48 software provides instrument control, data collection, and data analysis. The most common qPCR parameters are automatically in place. These can be easily changed on the computer to meet specific experimental requirements.

Features

  • MIQE compliant open licence software giving confidence when it comes to publishing your data.
  • HRM functionality as standard: with the ability to discriminate class IV SNP >99% of the time.
  • Eco 48 allows for simple multiplex analysis with up to four dyes at once.
  • World most thermally accurate block gives consistent data every time.
  • High uniformity provides high quality data. Fast cycling enables several experiments per day, all at an economical price.
  • Fastest block-based real-time PCR system. 40 cycles in 40 minutes standardly and 40 cycles in as little as 15 minutes after optimisation of temperatures and hold times.
  • Eco 48 is an open platform that can utilise any chemistry, dye or PCR reagent.
  • Easy to use software, making analysis simple and fast.
  • Industry-leading data quality.
  • Eco 48 format provides a small 34.5cm x 31cm (13.6” × 12.2”) footprint to save bench spaceparameters are automatically in place. These can be easily changed on the computer to meet specific experimental requirements.

ECO 48 Thermal Technology

qPCR specificity and efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the entire heat block, ensuring that all samples are processed equally. The patented hollow silver and gold liquid filled thermal block design of Eco 48 achieves this with a unique heating and cooling system that provides accurate ±0.1ºC (measured at 95 ºC) temperature control and quickly cycles from one temperature to the next not requiring any settle time at all to hit your specified temperatures.

ECO 48 Optical System

The Eco 48 Real Time PCR system contains an advanced high-performance optical system that delivers precise and sensitive fluorescence detection, facilitating all 4 colour multiplex applications. The system is provided factory-calibrated for use with SYBR®, FAM™, HEX®, VIC®, ROX®, Cy®5 and can also be used with any qPCR fluorophores overlapping with these calibrated dyes. For excitation, two panels of 48 fixed LEDs provide excitation energy of distinct spectra, enabling broad range excitation. Each of the 48 LEDs illuminates a specific plate well location, eliminating the optical distortion created by most stationary optical systems.

HRM analysis protocols are supported by continuous data acquisition during the melt for increased data collection and reduced run times. 

 

The user can change the plate setup and perform data analysis after the run is complete.

Rapid Cycling

Eco 48 has rapid heating and cooling rates. Average ramp rates of 5.5°C/sec to aid in reduction of overall PCR run times. The typical run time for a 40 cycle PCR protocol is around 40 minutes. In testing, the quickest optimised programme enabled a 40 cycle qPCR experiment to complete in just under 20 minutes without sacrificing thermal uniformity in the process as the block still achieves a thermal uniformity of ±0.1ºC (measured at 95 ºC) at these speeds.

High Thoroughput 

Eco 48 thermal control is superior to traditional 96-well peltier systems where thermal efficiency decreases as distance from the peltier increases. With Eco 48, faster and more precise cycling increases sample throughput because the need for multiple replicates is reduced by increased uniformity across the block.

Eco 48’s well-to-well, plate-to-plate and instrument-to-instrument results are as consistent as running a single experiment. To take advantage of this, Eco 48 has a function in the software where different experiments are reliably combined and analysed as one. High uniformity reduces the need for experimental replicates which saves on precious samples, eliminates the need for more expensive instruments, reduces reagent running costs and ensures you can do more with the sample you have.

Software

The Eco 48 system come with two distinct software’s, the first, Eco Control, drives the units set up and experimental runs while Eco Study is the analysis package.

The Eco 48 softwares are both open licence, you can install them on as many PCs as you like, and MIQE compliant.

  • Eco 48 software uses a unique icon-driven user interface to simplify experimental design and setup.
  • Pre-set thermal profile defaults are provided for the most commonly used experimental protocols.
  • Temperature and time for each protocol step can easily be changed by click-and-drag action.
  • Experiment templates can be customized and saved for future use.
  • With the Eco 48 system and software, data collection is monitored in real time, allowing researchers to access run viability immediately.
  • The user-friendly data analysis interface also allows researchers to easily view the amplification plot, melt curve analysis, and the analysed results, including Cq values, PCR efficiency, R2 and Y-intercept.
  • Data can be exported into Excel or CSV and custom reports generated directly into PowerPoint or PDF formats.
  • High-resolution images can be directly exported in multiple image formats, ready to use in any presentation.
  • Conforms to Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, making data analysis and submission for publication review more efficient.

ECO Control Software

  • User-installable, intuitive, easy to use software that integrates user control, real-time data collection, and advanced data analysis.
  • Supplied fully featured including HRM capability.
  • New software enables a widened chemistry library and contains new thermal profiles.

ECO Study Software

  • Data analysis and Multi-Experiment Analysis.
  • Allows higher throughput, pool separate plates together and analyse as one experiment.
  • Almost limitless sample volume.
  • Supplied on a USB stick

 


Availability: IN STOCK
SKU# PRIMEPRO48

Prime Pro 48 Real Time PCR System, including Pro Dock and software (Control and Study). PC not supplied

The Prime Pro 48 real time qPCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.

High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimised 40 cycle reaction down to only 15 minutes. qPCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a
unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.

The capacity of Prime Pro 48 accommodates a unique and economical 48-well qPCR plate. The well format mirrors a 384-well plate therefore allowing the use of 16-channel pipettes. The small qPCR plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity and greatly reduces run times.


Key benefits

Speed
  • 40 cycles in 40 minutes before optimisation.
  • 40 cycles in 15 minutes when optimised.
  • Genotype in 4 minutes with over 99% accuracy.
  • Set up a 4plex reaction in only 4 mouse clicks.

Confidence
  • MIQE compliant - always consistent, always compliant.
  •  Instant settle time to achieve world leading uniformity of ±0.1°C across the block - any well, any instrument - consistent results in any system.
  • 400 analyte specific qPCR reagent kits available.
  • Demo equipment available to evaluate in your own lab.

Performance
  • Consistent results provide trust and confidence. Well-to-well, run-to-run , machine-to-machine.
  • Compile data from multiple experiments. Pool plates and results across labs and instruments.
  • Record every well, with every filter, in every cycle. Never miss a data point.
  • Adaptive LED Control means you never have uncertainty of saturated unknowns, no bleaching though light to neighbouring wells or maximising the wide dynamic range. Trust results are correct. 
  • All other block-based qPCR machines have lower uniformity. If you perform Next Generation Sequencing, any other instrument than Prime Pro 48 is risking your NGS, with significant cost implications.

Value
  • No other qPCR system offers so much performance for such a low price.
  • Available as a complete system - Techne instrument, plates, seals and reagents.
  • Alternatively, is chemically open platform - use your preferred supplier reagents.
  • Lowest price per run - use duplicates and low sample volumes. Don't waste money with triplicates and large sample volumes.
  • Everything as standard - no add-ons, HRM as standard, unlimited software licence. 
  • Highly accurate and uniform qPCR prevents means successful NGS runs and significant moneysaving.

Sensitivity
  • ±0.1°C uniformity across the whole block instantly after every temperature change means that any well, in any instrument, provides same answer
  • HRM - Identify Class IV SNP >99.9% of the time
  • Unsurpassed block uniformity with patented block technology enables the researcher to use duplicates on Prime Pro 48, whereas triplicates are required on less uniform competitor machines.


Technical Specification


High Resolution Melt Yes
Volume per well Validated for 5 to 20 ul
Detection sensitivity 1 copy
Temp uniformity +- 0.1°C
Temp accuracy +- 0.15°C @ 60°C and 95°C
Temperature range 30 to 100 degC
Average ramp rate 5.5 degC sec
Thermal system Hollow silver block peltier-based with conductive fluid
Block format 48-well block
Consumables 48-well custom plates and optical adhesive seals
Optical system Dual LED excitation (452 to 486 nm and 542 to 582 nm)
Camera CCD camera
Filters 4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)
Calibrated dyes SYBR FAM HEX ROX Cy5
Additional dyes All dyes within range compatible with no additional calibration
Passive reference dyes Use of ROX is supported but optional
Data collection Data always collected in all four filters for all wells
Plate setup Plate setup for data analysis can be altered after run completes
Melt curve Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
PCR cycle time (unoptimised) 40 cycles in less than 40 minutes
PCR cycle time (FAST optimised) 40 cycles in less than 20 minutes
Dynamic range Greater than 9 logs
Calibration Not required
Installation Plug and play design
Precision Discriminates 5000 and 10000 template copies with 99 percent confidence
Warranty 1 year (parts and labour included)
Voltage 100 to 240 V.AC
Frequency 50 and 60 Hz
Nominal current draw 5A
Peak power 500W (typical power is 180W)
Software Multiple license software included at no additional cost
Chemistry All chemistries supported
Applications Absolute Quantification and Relative Quantification
Applications Allelic Discrimination and High Resolution Melt
Dimensions closed (WxDxH) 34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)
Dimensions open (WxDxH) 34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)
Weight 13.6 Kg (30 lb)
 

 

 


Availability: IN STOCK